Number of amino acids: 1255
Molecular weight: 139109.1
Theoretical pI: 5.56
Amino acid composition:
Ala (A) 85 6.8%
Arg (R) 39 3.1%
Asn (N) 81 6.5%
Asp (D) 73 5.8%
Cys (C) 39 3.1%
Gln (Q) 55 4.4%
Glu (E) 42 3.3%
Gly (G) 79 6.3%
His (H) 15 1.2%
Ile (I) 78 6.2%
Leu (L) 99 7.9%
Lys (K) 60 4.8%
Met (M) 20 1.6%
Phe (F) 83 6.6%
Pro (P) 57 4.5%
Ser (S) 95 7.6%
Thr (T) 99 7.9%
Trp (W) 11 0.9%
Tyr (Y) 54 4.3%
Val (V) 91 7.3%
Asx (B) 0 0.0%
Glx (Z) 0 0.0%
Xaa (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 115
Total number of positively charged residues (Arg + Lys): 99
Atomic composition:
Carbon C 6252
Hydrogen H 9593
Nitrogen N 1609
Oxygen O 1870
Sulfur S 59
Formula: C6252H9593N1609O1870S59
Total number of atoms: 19383
Extinction coefficients:
Conditions: 6.0 M guanidium hydrochloride
0.02 M phosphate buffer
pH 6.5
Extinction coefficients are in units of M-1 cm-1 .
The first table lists values computed assuming ALL Cys
residues appear as half cystines, whereas the second table
assumes that NONE do.
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 140455 139613 137170 133990 128680
Abs 0.1% (=1 g/l) 1.010 1.004 0.986 0.963 0.925
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 137700 137200 134890 131710 126400
Abs 0.1% (=1 g/l) 0.990 0.986 0.970 0.947 0.909
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 32.42
This classifies the protein as stable.
Aliphatic index: 82.80
Grand average of hydropathicity (GRAVY): -0.043
Protein Sequence Feature:
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