Name: putative E2 glycoprotein precursor; putative spike glycoprotein [SARS coronavirus] Source Data:
(From: http://us.expasy.org)

Physical Attributes:

 

Number of amino acids: 1255

Molecular weight: 139109.1

Theoretical pI: 5.56

Amino acid composition:

Ala (A)  85	  6.8%
Arg (R)  39	  3.1%
Asn (N)  81	  6.5%
Asp (D)  73	  5.8%
Cys (C)  39	  3.1%
Gln (Q)  55	  4.4%
Glu (E)  42	  3.3%
Gly (G)  79	  6.3%
His (H)  15	  1.2%
Ile (I)  78	  6.2%
Leu (L)  99	  7.9%
Lys (K)  60	  4.8%
Met (M)  20	  1.6%
Phe (F)  83	  6.6%
Pro (P)  57	  4.5%
Ser (S)  95	  7.6%
Thr (T)  99	  7.9%
Trp (W)  11	  0.9%
Tyr (Y)  54	  4.3%
Val (V)  91	  7.3%

Asx (B)   0	  0.0%
Glx (Z)   0	  0.0%
Xaa (X)   0	  0.0%

Total number of negatively charged residues (Asp + Glu): 115
Total number of positively charged residues (Arg + Lys): 99

Atomic composition:

Carbon      C	      6252
Hydrogen    H	      9593
Nitrogen    N	      1609
Oxygen      O	      1870
Sulfur      S	        59

Formula: C6252H9593N1609O1870S59
Total number of atoms: 19383

Extinction coefficients:

Conditions: 6.0 M guanidium hydrochloride
            0.02 M phosphate buffer
            pH 6.5

Extinction coefficients are in units of  M-1 cm-1 .

The first table lists values computed assuming ALL Cys 
residues appear as half cystines, whereas the second table 
assumes that NONE do. 

                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient   140455  139613  137170  133990  128680
Abs 0.1% (=1 g/l)   1.010   1.004   0.986   0.963   0.925



                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient   137700  137200  134890  131710  126400
Abs 0.1% (=1 g/l)   0.990   0.986   0.970   0.947   0.909


Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 32.42
This classifies the protein as stable.



Aliphatic index: 82.80

Grand average of hydropathicity (GRAVY): -0.043 
 
            

Protein Sequence Feature:
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2003-4-26