Number of amino acids: 274

Molecular weight: 30902.7

Theoretical pI: 5.75

Amino acid composition:

Ala (A)  20	  7.3%
Arg (R)   5	  1.8%
Asn (N)  12	  4.4%
Asp (D)  14	  5.1%
Cys (C)   8	  2.9%
Gln (Q)  11	  4.0%
Glu (E)   8	  2.9%
Gly (G)  14	  5.1%
His (H)   7	  2.6%
Ile (I)  22	  8.0%
Leu (L)  27	  9.9%
Lys (K)  12	  4.4%
Met (M)   5	  1.8%
Phe (F)  16	  5.8%
Pro (P)  13	  4.7%
Ser (S)  15	  5.5%
Thr (T)  23	  8.4%
Trp (W)   5	  1.8%
Tyr (Y)  17	  6.2%
Val (V)  20	  7.3%

Asx (B)   0	  0.0%
Glx (Z)   0	  0.0%
Xaa (X)   0	  0.0%

Total number of negatively charged residues (Asp + Glu): 22
Total number of positively charged residues (Arg + Lys): 17

Atomic composition:

Carbon      C	      1428
Hydrogen    H	      2161
Nitrogen    N	       343
Oxygen      O	       397
Sulfur      S	        13

Formula: C1428H2161N343O397S13
Total number of atoms: 4342

Extinction coefficients:

Conditions: 6.0 M guanidium hydrochloride
            0.02 M phosphate buffer
            pH 6.5

Extinction coefficients are in units of  M-1 cm-1 .

The first table lists values computed assuming ALL Cys 
residues appear as half cystines, whereas the second table 
assumes that NONE do. 

                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    52230   52308   51645   50690   48880
Abs 0.1% (=1 g/l)   1.690   1.693   1.671   1.640   1.582



                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    51650   51800   51165   50210   48400
Abs 0.1% (=1 g/l)   1.671   1.676   1.656   1.625   1.566


Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.04
This classifies the protein as stable.



Aliphatic index: 98.21

Grand average of hydropathicity (GRAVY): 0.239