putative
small envelope protein E [SARS coronavirus]
Source Data: (From: http://us.expasy.org)
Physical Attributes:
Molecular weight: 8361.0
Theoretical pI: 6.01
Amino acid composition:
Ala (A) 4 5.3%
Arg (R) 2 2.6%
Asn (N) 5 6.6%
Asp (D) 1 1.3%
Cys (C) 3 3.9%
Gln (Q) 0 0.0%
Glu (E) 3 3.9%
Gly (G) 2 2.6%
His (H) 0 0.0%
Ile (I) 3 3.9%
Leu (L) 14 18.4%
Lys (K) 2 2.6%
Met (M) 1 1.3%
Phe (F) 4 5.3%
Pro (P) 2 2.6%
Ser (S) 7 9.2%
Thr (T) 5 6.6%
Trp (W) 0 0.0%
Tyr (Y) 4 5.3%
Val (V) 14 18.4%
Asx (B) 0 0.0%
Glx (Z) 0 0.0%
Xaa (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 4
Total number of positively charged residues (Arg + Lys): 4
Atomic composition:
Carbon C 388
Hydrogen H 625
Nitrogen N 89
Oxygen O 106
Sulfur S 4
Formula: C388H625N89O106S4
Total number of atoms: 1212
Extinction coefficients:
Conditions: 6.0 M guanidium hydrochloride
0.02 M phosphate buffer
pH 6.5
Extinction coefficients are in units of M-1 cm-1 .
The first table lists values computed assuming ALL Cys
residues appear as half cystines, whereas the second table
assumes that NONE do.
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 5945 5727 5500 5240 4920
Abs 0.1% (=1 g/l) 0.711 0.685 0.658 0.627 0.588
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 5800 5600 5380 5120 4800
Abs 0.1% (=1 g/l) 0.694 0.670 0.643 0.612 0.574
Estimated half-life:
The N-terminal of the sequence considered is M (Met).
The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 30.48
This classifies the protein as stable.
Aliphatic index: 145.92
Grand average of hydropathicity (GRAVY): 1.141
Protein Sequence Feature:
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