Number of amino acids: 221

Molecular weight: 25060.5

Theoretical pI: 9.63

Amino acid composition:

Ala (A)  19	  8.6%
Arg (R)  15	  6.8%
Asn (N)  13	  5.9%
Asp (D)   6	  2.7%
Cys (C)   3	  1.4%
Gln (Q)   5	  2.3%
Glu (E)   7	  3.2%
Gly (G)  15	  6.8%
His (H)   3	  1.4%
Ile (I)  18	  8.1%
Leu (L)  31	 14.0%
Lys (K)   6	  2.7%
Met (M)   7	  3.2%
Phe (F)  11	  5.0%
Pro (P)   5	  2.3%
Ser (S)  12	  5.4%
Thr (T)  13	  5.9%
Trp (W)   7	  3.2%
Tyr (Y)   9	  4.1%
Val (V)  16	  7.2%

Asx (B)   0	  0.0%
Glx (Z)   0	  0.0%
Xaa (X)   0	  0.0%

Total number of negatively charged residues (Asp + Glu): 13
Total number of positively charged residues (Arg + Lys): 21

Atomic composition:

Carbon      C	      1155
Hydrogen    H	      1809
Nitrogen    N	       303
Oxygen      O	       300
Sulfur      S	        10

Formula: C1155H1809N303O300S10
Total number of atoms: 3577

Extinction coefficients:

Conditions: 6.0 M guanidium hydrochloride
            0.02 M phosphate buffer
            pH 6.5

Extinction coefficients are in units of  M-1 cm-1 .

The first table lists values computed assuming ALL Cys 
residues appear as half cystines, whereas the second table 
assumes that NONE do. 

                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    50995   51927   51845   51470   50120
Abs 0.1% (=1 g/l)   2.035   2.072   2.069   2.054   2.000



                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    50850   51800   51725   51350   50000
Abs 0.1% (=1 g/l)   2.029   2.067   2.064   2.049   1.995


Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 30.44
This classifies the protein as stable.



Aliphatic index: 116.06

Grand average of hydropathicity (GRAVY): 0.417