Number of amino acids: 422

Molecular weight: 46025.0

Theoretical pI: 10.11

Amino acid composition:

Ala (A)  34	  8.1%
Arg (R)  31	  7.3%
Asn (N)  25	  5.9%
Asp (D)  22	  5.2%
Cys (C)   0	  0.0%
Gln (Q)  34	  8.1%
Glu (E)  14	  3.3%
Gly (G)  45	 10.7%
His (H)   5	  1.2%
Ile (I)  11	  2.6%
Leu (L)  26	  6.2%
Lys (K)  29	  6.9%
Met (M)   7	  1.7%
Phe (F)  13	  3.1%
Pro (P)  31	  7.3%
Ser (S)  35	  8.3%
Thr (T)  33	  7.8%
Trp (W)   5	  1.2%
Tyr (Y)  11	  2.6%
Val (V)  11	  2.6%

Asx (B)   0	  0.0%
Glx (Z)   0	  0.0%
Xaa (X)   0	  0.0%

Total number of negatively charged residues (Asp + Glu): 36
Total number of positively charged residues (Arg + Lys): 60

Atomic composition:

Carbon      C	      1985
Hydrogen    H	      3150
Nitrogen    N	       618
Oxygen      O	       633
Sulfur      S	         7

Formula: C1985H3150N618O633S7
Total number of atoms: 6393

Extinction coefficients:

Conditions: 6.0 M guanidium hydrochloride
            0.02 M phosphate buffer
            pH 6.5

Extinction coefficients are in units of  M-1 cm-1 .

                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    42950   43400   43095   42530   41200
Abs 0.1% (=1 g/l)   0.933   0.943   0.936   0.924   0.895


Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 52.28
This classifies the protein as unstable.



Aliphatic index: 49.81

Grand average of hydropathicity (GRAVY): -1.027