Name: putative leader protein [SARS coronavirus] Source Data:
(From: http://us.expasy.org)

Physical Attributes:

 

Number of amino acids: 179

Molecular weight: 19583.0

Theoretical pI: 5.35

Amino acid composition:

Ala (A)   7	  3.9%
Arg (R)   9	  5.0%
Asn (N)   9	  5.0%
Asp (D)   9	  5.0%
Cys (C)   1	  0.6%
Gln (Q)   6	  3.4%
Glu (E)  17	  9.5%
Gly (G)  22	 12.3%
His (H)   7	  3.9%
Ile (I)   6	  3.4%
Leu (L)  24	 13.4%
Lys (K)   9	  5.0%
Met (M)   2	  1.1%
Phe (F)   2	  1.1%
Pro (P)   6	  3.4%
Ser (S)  10	  5.6%
Thr (T)   8	  4.5%
Trp (W)   1	  0.6%
Tyr (Y)   6	  3.4%
Val (V)  18	 10.1%

Asx (B)   0	  0.0%
Glx (Z)   0	  0.0%
Xaa (X)   0	  0.0%

Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 18

Atomic composition:

Carbon      C	       860
Hydrogen    H	      1379
Nitrogen    N	       245
Oxygen      O	       271
Sulfur      S	         3

Formula: C860H1379N245O271S3
Total number of atoms: 2758

Extinction coefficients:

Conditions: 6.0 M guanidium hydrochloride
            0.02 M phosphate buffer
            pH 6.5

Extinction coefficients are in units of  M-1 cm-1 .

The first table lists values computed assuming ALL Cys 
residues appear as half cystines, whereas the second table 
assumes that NONE do. 

                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    14100   14000   13730   13370   12800
Abs 0.1% (=1 g/l)   0.720   0.715   0.701   0.683   0.654



                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    14100   14000   13730   13370   12800
Abs 0.1% (=1 g/l)   0.720   0.715   0.701   0.683   0.654


Estimated half-life:

The N-terminal of the sequence considered is M (Met).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 26.71
This classifies the protein as stable.



Aliphatic index: 98.44

Grand average of hydropathicity (GRAVY): -0.357 
 
            

Protein Sequence Feature:
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2003-4-26