Number of amino acids: 639

Molecular weight: 70679.1

Theoretical pI: 6.08

Amino acid composition:

Ala (A)  41	  6.4%
Arg (R)  26	  4.1%
Asn (N)  29	  4.5%
Asp (D)  33	  5.2%
Cys (C)  26	  4.1%
Gln (Q)  22	  3.4%
Glu (E)  43	  6.7%
Gly (G)  47	  7.4%
His (H)  13	  2.0%
Ile (I)  41	  6.4%
Leu (L)  57	  8.9%
Lys (K)  43	  6.7%
Met (M)  10	  1.6%
Phe (F)  27	  4.2%
Pro (P)  25	  3.9%
Ser (S)  41	  6.4%
Thr (T)  40	  6.3%
Trp (W)   7	  1.1%
Tyr (Y)  17	  2.7%
Val (V)  51	  8.0%

Asx (B)   0	  0.0%
Glx (Z)   0	  0.0%
Xaa (X)   0	  0.0%

Total number of negatively charged residues (Asp + Glu): 76
Total number of positively charged residues (Arg + Lys): 69

Atomic composition:

Carbon      C	      3134
Hydrogen    H	      4966
Nitrogen    N	       844
Oxygen      O	       941
Sulfur      S	        36

Formula: C3134H4966N844O941S36
Total number of atoms: 9921

Extinction coefficients:

Conditions: 6.0 M guanidium hydrochloride
            0.02 M phosphate buffer
            pH 6.5

Extinction coefficients are in units of  M-1 cm-1 .

The first table lists values computed assuming ALL Cys 
residues appear as half cystines, whereas the second table 
assumes that NONE do. 

                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    64335   64651   64045   63150   61160
Abs 0.1% (=1 g/l)   0.910   0.915   0.906   0.893   0.865



                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    62450   63000   62485   61590   59600
Abs 0.1% (=1 g/l)   0.884   0.891   0.884   0.871   0.843


Estimated half-life:

The N-terminal of the sequence considered is G (Gly).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 35.79
This classifies the protein as stable.



Aliphatic index: 89.37

Grand average of hydropathicity (GRAVY): -0.110