Number of amino acids: 2422

Molecular weight: 269724.7

Theoretical pI: 5.55

Amino acid composition:

Ala (A) 180	  7.4%
Arg (R)  71	  2.9%
Asn (N) 115	  4.7%
Asp (D) 123	  5.1%
Cys (C)  72	  3.0%
Gln (Q)  71	  2.9%
Glu (E) 146	  6.0%
Gly (G) 132	  5.5%
His (H)  49	  2.0%
Ile (I) 119	  4.9%
Leu (L) 232	  9.6%
Lys (K) 148	  6.1%
Met (M)  56	  2.3%
Phe (F) 115	  4.7%
Pro (P)  94	  3.9%
Ser (S) 177	  7.3%
Thr (T) 186	  7.7%
Trp (W)  23	  0.9%
Tyr (Y) 114	  4.7%
Val (V) 199	  8.2%

Asx (B)   0	  0.0%
Glx (Z)   0	  0.0%
Xaa (X)   0	  0.0%

Total number of negatively charged residues (Asp + Glu): 269
Total number of positively charged residues (Arg + Lys): 219

Atomic composition:

Carbon      C	     12105
Hydrogen    H	     18816
Nitrogen    N	      3090
Oxygen      O	      3624
Sulfur      S	       128

Formula: C12105H18816N3090O3624S128
Total number of atoms: 37763

Extinction coefficients:

Conditions: 6.0 M guanidium hydrochloride
            0.02 M phosphate buffer
            pH 6.5

Extinction coefficients are in units of  M-1 cm-1 .

The first table lists values computed assuming ALL Cys 
residues appear as half cystines, whereas the second table 
assumes that NONE do. 

                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient   294720  292972  287830  281110  269920
Abs 0.1% (=1 g/l)   1.093   1.086   1.067   1.042   1.001



                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient   289500  288400  283510  276790  265600
Abs 0.1% (=1 g/l)   1.073   1.069   1.051   1.026   0.985


Estimated half-life:

The N-terminal of the sequence considered is A (Ala).

The estimated half-life is: 4.4 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 37.27
This classifies the protein as stable.



Aliphatic index: 87.78

Grand average of hydropathicity (GRAVY): -0.043