Number of amino acids: 306

Molecular weight: 33845.7

Theoretical pI: 6.22

Amino acid composition:

Ala (A)  17	  5.6%
Arg (R)  12	  3.9%
Asn (N)  19	  6.2%
Asp (D)  17	  5.6%
Cys (C)  12	  3.9%
Gln (Q)  14	  4.6%
Glu (E)   9	  2.9%
Gly (G)  26	  8.5%
His (H)   8	  2.6%
Ile (I)  12	  3.9%
Leu (L)  30	  9.8%
Lys (K)  11	  3.6%
Met (M)  10	  3.3%
Phe (F)  16	  5.2%
Pro (P)  13	  4.2%
Ser (S)  16	  5.2%
Thr (T)  26	  8.5%
Trp (W)   3	  1.0%
Tyr (Y)  11	  3.6%
Val (V)  24	  7.8%

Asx (B)   0	  0.0%
Glx (Z)   0	  0.0%
Xaa (X)   0	  0.0%

Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 23

Atomic composition:

Carbon      C	      1499
Hydrogen    H	      2325
Nitrogen    N	       405
Oxygen      O	       445
Sulfur      S	        22

Formula: C1499H2325N405O445S22
Total number of atoms: 4696

Extinction coefficients:

Conditions: 6.0 M guanidium hydrochloride
            0.02 M phosphate buffer
            pH 6.5

Extinction coefficients are in units of  M-1 cm-1 .

The first table lists values computed assuming ALL Cys 
residues appear as half cystines, whereas the second table 
assumes that NONE do. 

                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    33020   32962   32495   31870   30720
Abs 0.1% (=1 g/l)   0.976   0.974   0.960   0.942   0.908



                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    32150   32200   31775   31150   30000
Abs 0.1% (=1 g/l)   0.950   0.951   0.939   0.920   0.886


Estimated half-life:

The N-terminal of the sequence considered is S (Ser).

The estimated half-life is: 1.9 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 29.67
This classifies the protein as stable.



Aliphatic index: 81.83

Grand average of hydropathicity (GRAVY): -0.049