Number of amino acids: 290

Molecular weight: 33023.7

Theoretical pI: 9.08

Amino acid composition:

Ala (A)  22	  7.6%
Arg (R)   9	  3.1%
Asn (N)   9	  3.1%
Asp (D)   7	  2.4%
Cys (C)  12	  4.1%
Gln (Q)   7	  2.4%
Glu (E)   4	  1.4%
Gly (G)  14	  4.8%
His (H)   4	  1.4%
Ile (I)  17	  5.9%
Leu (L)  45	 15.5%
Lys (K)  13	  4.5%
Met (M)  14	  4.8%
Phe (F)  19	  6.6%
Pro (P)   7	  2.4%
Ser (S)  16	  5.5%
Thr (T)  20	  6.9%
Trp (W)   6	  2.1%
Tyr (Y)  19	  6.6%
Val (V)  26	  9.0%

Asx (B)   0	  0.0%
Glx (Z)   0	  0.0%
Xaa (X)   0	  0.0%

Total number of negatively charged residues (Asp + Glu): 11
Total number of positively charged residues (Arg + Lys): 22

Atomic composition:

Carbon      C	      1548
Hydrogen    H	      2392
Nitrogen    N	       360
Oxygen      O	       384
Sulfur      S	        26

Formula: C1548H2392N360O384S26
Total number of atoms: 4710

Extinction coefficients:

Conditions: 6.0 M guanidium hydrochloride
            0.02 M phosphate buffer
            pH 6.5

Extinction coefficients are in units of  M-1 cm-1 .

The first table lists values computed assuming ALL Cys 
residues appear as half cystines, whereas the second table 
assumes that NONE do. 

                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    60820   60962   60235   59180   57120
Abs 0.1% (=1 g/l)   1.842   1.846   1.824   1.792   1.730



                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    59950   60200   59515   58460   56400
Abs 0.1% (=1 g/l)   1.815   1.823   1.802   1.770   1.708


Estimated half-life:

The N-terminal of the sequence considered is G (Gly).

The estimated half-life is: 30 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 22.56
This classifies the protein as stable.



Aliphatic index: 116.97

Grand average of hydropathicity (GRAVY): 0.807