Number of amino acids: 83

Molecular weight: 9267.8

Theoretical pI: 5.18

Amino acid composition:

Ala (A)   5	  6.0%
Arg (R)   3	  3.6%
Asn (N)   3	  3.6%
Asp (D)   5	  6.0%
Cys (C)   3	  3.6%
Gln (Q)   6	  7.2%
Glu (E)   5	  6.0%
Gly (G)   1	  1.2%
His (H)   1	  1.2%
Ile (I)   2	  2.4%
Leu (L)  15	 18.1%
Lys (K)   5	  6.0%
Met (M)   4	  4.8%
Phe (F)   1	  1.2%
Pro (P)   0	  0.0%
Ser (S)  10	 12.0%
Thr (T)   4	  4.8%
Trp (W)   1	  1.2%
Tyr (Y)   0	  0.0%
Val (V)   9	 10.8%

Asx (B)   0	  0.0%
Glx (Z)   0	  0.0%
Xaa (X)   0	  0.0%

Total number of negatively charged residues (Asp + Glu): 10
Total number of positively charged residues (Arg + Lys): 8

Atomic composition:

Carbon      C	       400
Hydrogen    H	       675
Nitrogen    N	       109
Oxygen      O	       127
Sulfur      S	         7

Formula: C400H675N109O127S7
Total number of atoms: 1318

Extinction coefficients:

Conditions: 6.0 M guanidium hydrochloride
            0.02 M phosphate buffer
            pH 6.5

Extinction coefficients are in units of  M-1 cm-1 .

The first table lists values computed assuming ALL Cys 
residues appear as half cystines, whereas the second table 
assumes that NONE do. 

                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient     5545    5727    5780    5810    5720
Abs 0.1% (=1 g/l)   0.598   0.618   0.624   0.627   0.617



                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient     5400    5600    5660    5690    5600
Abs 0.1% (=1 g/l)   0.583   0.604   0.611   0.614   0.604


Estimated half-life:

The N-terminal of the sequence considered is S (Ser).

The estimated half-life is: 1.9 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 50.14
This classifies the protein as unstable.



Aliphatic index: 117.35

Grand average of hydropathicity (GRAVY): 0.192