Number of amino acids: 198

Molecular weight: 21866.0

Theoretical pI: 6.58

Amino acid composition:

Ala (A)  28	 14.1%
Arg (R)   7	  3.5%
Asn (N)  14	  7.1%
Asp (D)  13	  6.6%
Cys (C)   2	  1.0%
Gln (Q)  11	  5.6%
Glu (E)   9	  4.5%
Gly (G)   4	  2.0%
His (H)   0	  0.0%
Ile (I)   9	  4.5%
Leu (L)  18	  9.1%
Lys (K)  15	  7.6%
Met (M)   9	  4.5%
Phe (F)   4	  2.0%
Pro (P)   6	  3.0%
Ser (S)  14	  7.1%
Thr (T)  12	  6.1%
Trp (W)   2	  1.0%
Tyr (Y)   7	  3.5%
Val (V)  14	  7.1%

Asx (B)   0	  0.0%
Glx (Z)   0	  0.0%
Xaa (X)   0	  0.0%

Total number of negatively charged residues (Asp + Glu): 22
Total number of positively charged residues (Arg + Lys): 22

Atomic composition:

Carbon      C	       956
Hydrogen    H	      1547
Nitrogen    N	       261
Oxygen      O	       301
Sulfur      S	        11

Formula: C956H1547N261O301S11
Total number of atoms: 3076

Extinction coefficients:

Conditions: 6.0 M guanidium hydrochloride
            0.02 M phosphate buffer
            pH 6.5

Extinction coefficients are in units of  M-1 cm-1 .

The first table lists values computed assuming ALL Cys 
residues appear as half cystines, whereas the second table 
assumes that NONE do. 

                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    21095   21127   20855   20460   19720
Abs 0.1% (=1 g/l)   0.965   0.966   0.954   0.936   0.902



                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    20950   21000   20735   20340   19600
Abs 0.1% (=1 g/l)   0.958   0.960   0.948   0.930   0.896


Estimated half-life:

The N-terminal of the sequence considered is A (Ala).

The estimated half-life is: 4.4 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 39.23
This classifies the protein as stable.



Aliphatic index: 87.83

Grand average of hydropathicity (GRAVY): -0.226