Number of amino acids: 113

Molecular weight: 12401.1

Theoretical pI: 9.10

Amino acid composition:

Ala (A)  10	  8.8%
Arg (R)   6	  5.3%
Asn (N)   8	  7.1%
Asp (D)   6	  5.3%
Cys (C)   3	  2.7%
Gln (Q)   4	  3.5%
Glu (E)   3	  2.7%
Gly (G)   9	  8.0%
His (H)   1	  0.9%
Ile (I)   2	  1.8%
Leu (L)  14	 12.4%
Lys (K)   7	  6.2%
Met (M)   2	  1.8%
Phe (F)   4	  3.5%
Pro (P)   6	  5.3%
Ser (S)   6	  5.3%
Thr (T)  10	  8.8%
Trp (W)   1	  0.9%
Tyr (Y)   5	  4.4%
Val (V)   6	  5.3%

Asx (B)   0	  0.0%
Glx (Z)   0	  0.0%
Xaa (X)   0	  0.0%

Total number of negatively charged residues (Asp + Glu): 9
Total number of positively charged residues (Arg + Lys): 13

Atomic composition:

Carbon      C	       548
Hydrogen    H	       869
Nitrogen    N	       153
Oxygen      O	       165
Sulfur      S	         5

Formula: C548H869N153O165S5
Total number of atoms: 1740

Extinction coefficients:

Conditions: 6.0 M guanidium hydrochloride
            0.02 M phosphate buffer
            pH 6.5

Extinction coefficients are in units of  M-1 cm-1 .

The first table lists values computed assuming ALL Cys 
residues appear as half cystines, whereas the second table 
assumes that NONE do. 

                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    12795   12727   12505   12210   11720
Abs 0.1% (=1 g/l)   1.032   1.026   1.008   0.985   0.945



                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    12650   12600   12385   12090   11600
Abs 0.1% (=1 g/l)   1.020   1.016   0.999   0.975   0.935


Estimated half-life:

The N-terminal of the sequence considered is N (Asn).

The estimated half-life is: 1.4 hours (mammalian reticulocytes, in vitro).
                            3 min (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 32.03
This classifies the protein as stable.



Aliphatic index: 79.47

Grand average of hydropathicity (GRAVY): -0.314