putative
coronavirus nsp7; formerly known as growth-factor-like protein [SARS
coronavirus]
Source Data: (From: http://us.expasy.org)
Physical Attributes:
Number of amino acids: 139
Molecular weight: 14843.9
Theoretical pI: 6.30
Amino acid composition:
Ala (A) 12 8.6%
Arg (R) 3 2.2%
Asn (N) 7 5.0%
Asp (D) 7 5.0%
Cys (C) 13 9.4%
Gln (Q) 6 4.3%
Glu (E) 4 2.9%
Gly (G) 13 9.4%
His (H) 3 2.2%
Ile (I) 4 2.9%
Leu (L) 8 5.8%
Lys (K) 7 5.0%
Met (M) 4 2.9%
Phe (F) 5 3.6%
Pro (P) 9 6.5%
Ser (S) 6 4.3%
Thr (T) 13 9.4%
Trp (W) 1 0.7%
Tyr (Y) 5 3.6%
Val (V) 9 6.5%
Asx (B) 0 0.0%
Glx (Z) 0 0.0%
Xaa (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 11
Total number of positively charged residues (Arg + Lys): 10
Atomic composition:
Carbon C 638
Hydrogen H 993
Nitrogen N 175
Oxygen O 199
Sulfur S 17
Formula: C638H993N175O199S17
Total number of atoms: 2022
Extinction coefficients:
Conditions: 6.0 M guanidium hydrochloride
0.02 M phosphate buffer
pH 6.5
Extinction coefficients are in units of M-1 cm-1 .
The first table lists values computed assuming ALL Cys
residues appear as half cystines, whereas the second table
assumes that NONE do.
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 13520 13362 13105 12810 12320
Abs 0.1% (=1 g/l) 0.911 0.900 0.883 0.863 0.830
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 12650 12600 12385 12090 11600
Abs 0.1% (=1 g/l) 0.852 0.849 0.834 0.814 0.781
Estimated half-life:
The N-terminal of the sequence considered is A (Ala).
The estimated half-life is: 4.4 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 36.49
This classifies the protein as stable.
Aliphatic index: 61.08
Grand average of hydropathicity (GRAVY): -0.096
Protein Sequence Feature:
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