Number of amino acids: 932
Molecular weight: 106487.2
Theoretical pI: 6.01
Amino acid composition:
Ala (A) 69 7.4%
Arg (R) 43 4.6%
Asn (N) 56 6.0%
Asp (D) 73 7.8%
Cys (C) 31 3.3%
Gln (Q) 27 2.9%
Glu (E) 34 3.6%
Gly (G) 46 4.9%
His (H) 28 3.0%
Ile (I) 32 3.4%
Leu (L) 84 9.0%
Lys (K) 49 5.3%
Met (M) 27 2.9%
Phe (F) 55 5.9%
Pro (P) 30 3.2%
Ser (S) 50 5.4%
Thr (T) 55 5.9%
Trp (W) 9 1.0%
Tyr (Y) 58 6.2%
Val (V) 76 8.2%
Asx (B) 0 0.0%
Glx (Z) 0 0.0%
Xaa (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 107
Total number of positively charged residues (Arg + Lys): 92
Atomic composition:
Carbon C 4780
Hydrogen H 7250
Nitrogen N 1258
Oxygen O 1393
Sulfur S 58
Formula: C4780H7250N1258O1393S58
Total number of atoms: 14739
Extinction coefficients:
Conditions: 6.0 M guanidium hydrochloride
0.02 M phosphate buffer
pH 6.5
Extinction coefficients are in units of M-1 cm-1 .
The first table lists values computed assuming ALL Cys
residues appear as half cystines, whereas the second table
assumes that NONE do.
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 134875 133505 130750 127250 121800
Abs 0.1% (=1 g/l) 1.267 1.254 1.228 1.195 1.144
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 132700 131600 128950 125450 120000
Abs 0.1% (=1 g/l) 1.246 1.236 1.211 1.178 1.127
Estimated half-life:
The N-terminal of the sequence considered is S (Ser).
The estimated half-life is: 1.9 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 29.38
This classifies the protein as stable.
Aliphatic index: 79.59
Grand average of hydropathicity (GRAVY): -0.191
Protein Sequence Feature:
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