Name: putative coronavirus nsp9 (RdRp); RNA-dependent RNA polymerase [SARS coronavirus] Source Data:
(From: http://us.expasy.org)

Physical Attributes:

 

Number of amino acids: 932

Molecular weight: 106487.2

Theoretical pI: 6.01

Amino acid composition:

Ala (A)  69	  7.4%
Arg (R)  43	  4.6%
Asn (N)  56	  6.0%
Asp (D)  73	  7.8%
Cys (C)  31	  3.3%
Gln (Q)  27	  2.9%
Glu (E)  34	  3.6%
Gly (G)  46	  4.9%
His (H)  28	  3.0%
Ile (I)  32	  3.4%
Leu (L)  84	  9.0%
Lys (K)  49	  5.3%
Met (M)  27	  2.9%
Phe (F)  55	  5.9%
Pro (P)  30	  3.2%
Ser (S)  50	  5.4%
Thr (T)  55	  5.9%
Trp (W)   9	  1.0%
Tyr (Y)  58	  6.2%
Val (V)  76	  8.2%

Asx (B)   0	  0.0%
Glx (Z)   0	  0.0%
Xaa (X)   0	  0.0%

Total number of negatively charged residues (Asp + Glu): 107
Total number of positively charged residues (Arg + Lys): 92

Atomic composition:

Carbon      C	      4780
Hydrogen    H	      7250
Nitrogen    N	      1258
Oxygen      O	      1393
Sulfur      S	        58

Formula: C4780H7250N1258O1393S58
Total number of atoms: 14739

Extinction coefficients:

Conditions: 6.0 M guanidium hydrochloride
            0.02 M phosphate buffer
            pH 6.5

Extinction coefficients are in units of  M-1 cm-1 .

The first table lists values computed assuming ALL Cys 
residues appear as half cystines, whereas the second table 
assumes that NONE do. 

                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient   134875  133505  130750  127250  121800
Abs 0.1% (=1 g/l)   1.267   1.254   1.228   1.195   1.144



                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient   132700  131600  128950  125450  120000
Abs 0.1% (=1 g/l)   1.246   1.236   1.211   1.178   1.127


Estimated half-life:

The N-terminal of the sequence considered is S (Ser).

The estimated half-life is: 1.9 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 29.38
This classifies the protein as stable.



Aliphatic index: 79.59

Grand average of hydropathicity (GRAVY): -0.191 
 
            

Protein Sequence Feature:
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2003-4-26