Number of amino acids: 601

Molecular weight: 66868.7

Theoretical pI: 8.66

Amino acid composition:

Ala (A)  46	  7.7%
Arg (R)  30	  5.0%
Asn (N)  30	  5.0%
Asp (D)  29	  4.8%
Cys (C)  26	  4.3%
Gln (Q)  18	  3.0%
Glu (E)  23	  3.8%
Gly (G)  32	  5.3%
His (H)  12	  2.0%
Ile (I)  30	  5.0%
Leu (L)  50	  8.3%
Lys (K)  34	  5.7%
Met (M)   8	  1.3%
Phe (F)  24	  4.0%
Pro (P)  30	  5.0%
Ser (S)  40	  6.7%
Thr (T)  50	  8.3%
Trp (W)   3	  0.5%
Tyr (Y)  34	  5.7%
Val (V)  52	  8.7%

Asx (B)   0	  0.0%
Glx (Z)   0	  0.0%
Xaa (X)   0	  0.0%

Total number of negatively charged residues (Asp + Glu): 52
Total number of positively charged residues (Arg + Lys): 64

Atomic composition:

Carbon      C	      2982
Hydrogen    H	      4672
Nitrogen    N	       800
Oxygen      O	       878
Sulfur      S	        34

Formula: C2982H4672N800O878S34
Total number of atoms: 9366

Extinction coefficients:

Conditions: 6.0 M guanidium hydrochloride
            0.02 M phosphate buffer
            pH 6.5

Extinction coefficients are in units of  M-1 cm-1 .

The first table lists values computed assuming ALL Cys 
residues appear as half cystines, whereas the second table 
assumes that NONE do. 

                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    67385   66051   64270   62150   59160
Abs 0.1% (=1 g/l)   1.008   0.988   0.961   0.929   0.885



                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    65500   64400   62710   60590   57600
Abs 0.1% (=1 g/l)   0.980   0.963   0.938   0.906   0.861


Estimated half-life:

The N-terminal of the sequence considered is A (Ala).

The estimated half-life is: 4.4 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 33.45
This classifies the protein as stable.



Aliphatic index: 84.66

Grand average of hydropathicity (GRAVY): -0.095