Number of amino acids: 527

Molecular weight: 59894.6

Theoretical pI: 7.58

Amino acid composition:

Ala (A)  33	  6.3%
Arg (R)  22	  4.2%
Asn (N)  29	  5.5%
Asp (D)  33	  6.3%
Cys (C)  23	  4.4%
Gln (Q)  18	  3.4%
Glu (E)  18	  3.4%
Gly (G)  31	  5.9%
His (H)  21	  4.0%
Ile (I)  25	  4.7%
Leu (L)  38	  7.2%
Lys (K)  30	  5.7%
Met (M)  13	  2.5%
Phe (F)  30	  5.7%
Pro (P)  25	  4.7%
Ser (S)  28	  5.3%
Thr (T)  32	  6.1%
Trp (W)  10	  1.9%
Tyr (Y)  24	  4.6%
Val (V)  44	  8.3%

Asx (B)   0	  0.0%
Glx (Z)   0	  0.0%
Xaa (X)   0	  0.0%

Total number of negatively charged residues (Asp + Glu): 51
Total number of positively charged residues (Arg + Lys): 52

Atomic composition:

Carbon      C	      2692
Hydrogen    H	      4086
Nitrogen    N	       722
Oxygen      O	       761
Sulfur      S	        36

Formula: C2692H4086N722O761S36
Total number of atoms: 8297

Extinction coefficients:

Conditions: 6.0 M guanidium hydrochloride
            0.02 M phosphate buffer
            pH 6.5

Extinction coefficients are in units of  M-1 cm-1 .

The first table lists values computed assuming ALL Cys 
residues appear as half cystines, whereas the second table 
assumes that NONE do. 

                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    90395   90997   90200   88940   86120
Abs 0.1% (=1 g/l)   1.509   1.519   1.506   1.485   1.438



                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    88800   89600   88880   87620   84800
Abs 0.1% (=1 g/l)   1.483   1.496   1.484   1.463   1.416


Estimated half-life:

The N-terminal of the sequence considered is A (Ala).

The estimated half-life is: 4.4 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 29.36
This classifies the protein as stable.



Aliphatic index: 77.10

Grand average of hydropathicity (GRAVY): -0.183