Name: putative coronavirus nsp12 [SARS coronavirus] Source Data:
(From: http://us.expasy.org)

Physical Attributes:

 

Number of amino acids: 346

Molecular weight: 38491.9

Theoretical pI: 5.11

Amino acid composition:

Ala (A)  20	  5.8%
Arg (R)   9	  2.6%
Asn (N)  16	  4.6%
Asp (D)  24	  6.9%
Cys (C)   5	  1.4%
Gln (Q)  13	  3.8%
Glu (E)  22	  6.4%
Gly (G)  23	  6.6%
His (H)   7	  2.0%
Ile (I)  26	  7.5%
Leu (L)  28	  8.1%
Lys (K)  25	  7.2%
Met (M)   6	  1.7%
Phe (F)  18	  5.2%
Pro (P)  13	  3.8%
Ser (S)  23	  6.6%
Thr (T)  21	  6.1%
Trp (W)   3	  0.9%
Tyr (Y)  11	  3.2%
Val (V)  33	  9.5%

Asx (B)   0	  0.0%
Glx (Z)   0	  0.0%
Xaa (X)   0	  0.0%

Total number of negatively charged residues (Asp + Glu): 46
Total number of positively charged residues (Arg + Lys): 34

Atomic composition:

Carbon      C	      1733
Hydrogen    H	      2716
Nitrogen    N	       444
Oxygen      O	       523
Sulfur      S	        11

Formula: C1733H2716N444O523S11
Total number of atoms: 5427

Extinction coefficients:

Conditions: 6.0 M guanidium hydrochloride
            0.02 M phosphate buffer
            pH 6.5

Extinction coefficients are in units of  M-1 cm-1 .

The first table lists values computed assuming ALL Cys 
residues appear as half cystines, whereas the second table 
assumes that NONE do. 

                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    32440   32454   32015   31390   30240
Abs 0.1% (=1 g/l)   0.843   0.843   0.832   0.815   0.786



                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    32150   32200   31775   31150   30000
Abs 0.1% (=1 g/l)   0.835   0.837   0.825   0.809   0.779


Estimated half-life:

The N-terminal of the sequence considered is S (Ser).

The estimated half-life is: 1.9 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 34.88
This classifies the protein as stable.



Aliphatic index: 94.31

Grand average of hydropathicity (GRAVY): -0.089 
 
            

Protein Sequence Feature:
Note: click items to see a description. Drag two red cursors to select a zoom region.

All copyright reserved by CMBI©2003

2003-4-26