Number of amino acids: 346
Molecular weight: 38491.9
Theoretical pI: 5.11
Amino acid composition:
Ala (A) 20 5.8%
Arg (R) 9 2.6%
Asn (N) 16 4.6%
Asp (D) 24 6.9%
Cys (C) 5 1.4%
Gln (Q) 13 3.8%
Glu (E) 22 6.4%
Gly (G) 23 6.6%
His (H) 7 2.0%
Ile (I) 26 7.5%
Leu (L) 28 8.1%
Lys (K) 25 7.2%
Met (M) 6 1.7%
Phe (F) 18 5.2%
Pro (P) 13 3.8%
Ser (S) 23 6.6%
Thr (T) 21 6.1%
Trp (W) 3 0.9%
Tyr (Y) 11 3.2%
Val (V) 33 9.5%
Asx (B) 0 0.0%
Glx (Z) 0 0.0%
Xaa (X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 46
Total number of positively charged residues (Arg + Lys): 34
Atomic composition:
Carbon C 1733
Hydrogen H 2716
Nitrogen N 444
Oxygen O 523
Sulfur S 11
Formula: C1733H2716N444O523S11
Total number of atoms: 5427
Extinction coefficients:
Conditions: 6.0 M guanidium hydrochloride
0.02 M phosphate buffer
pH 6.5
Extinction coefficients are in units of M-1 cm-1 .
The first table lists values computed assuming ALL Cys
residues appear as half cystines, whereas the second table
assumes that NONE do.
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 32440 32454 32015 31390 30240
Abs 0.1% (=1 g/l) 0.843 0.843 0.832 0.815 0.786
276 278 279 280 282
nm nm nm nm nm
Ext. coefficient 32150 32200 31775 31150 30000
Abs 0.1% (=1 g/l) 0.835 0.837 0.825 0.809 0.779
Estimated half-life:
The N-terminal of the sequence considered is S (Ser).
The estimated half-life is: 1.9 hours (mammalian reticulocytes, in vitro).
>20 hours (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).
Instability index:
The instability index (II) is computed to be 34.88
This classifies the protein as stable.
Aliphatic index: 94.31
Grand average of hydropathicity (GRAVY): -0.089
Protein Sequence Feature:
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