Number of amino acids: 298

Molecular weight: 33580.6

Theoretical pI: 8.54

Amino acid composition:

Ala (A)  21	  7.0%
Arg (R)  10	  3.4%
Asn (N)  22	  7.4%
Asp (D)  16	  5.4%
Cys (C)   4	  1.3%
Gln (Q)  10	  3.4%
Glu (E)  10	  3.4%
Gly (G)  19	  6.4%
His (H)   6	  2.0%
Ile (I)  18	  6.0%
Leu (L)  31	 10.4%
Lys (K)  19	  6.4%
Met (M)  12	  4.0%
Phe (F)  11	  3.7%
Pro (P)  10	  3.4%
Ser (S)  21	  7.0%
Thr (T)  18	  6.0%
Trp (W)   7	  2.3%
Tyr (Y)  14	  4.7%
Val (V)  19	  6.4%

Asx (B)   0	  0.0%
Glx (Z)   0	  0.0%
Xaa (X)   0	  0.0%

Total number of negatively charged residues (Asp + Glu): 26
Total number of positively charged residues (Arg + Lys): 29

Atomic composition:

Carbon      C	      1511
Hydrogen    H	      2350
Nitrogen    N	       398
Oxygen      O	       436
Sulfur      S	        16

Formula: C1511H2350N398O436S16
Total number of atoms: 4711

Extinction coefficients:

Conditions: 6.0 M guanidium hydrochloride
            0.02 M phosphate buffer
            pH 6.5

Extinction coefficients are in units of  M-1 cm-1 .

The first table lists values computed assuming ALL Cys 
residues appear as half cystines, whereas the second table 
assumes that NONE do. 

                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    58390   59054   58690   57990   56240
Abs 0.1% (=1 g/l)   1.739   1.759   1.748   1.727   1.675



                      276     278     279     280     282
                       nm      nm      nm      nm      nm
Ext. coefficient    58100   58800   58450   57750   56000
Abs 0.1% (=1 g/l)   1.730   1.751   1.741   1.720   1.668


Estimated half-life:

The N-terminal of the sequence considered is A (Ala).

The estimated half-life is: 4.4 hours (mammalian reticulocytes, in vitro).
                            >20 hours (yeast, in vivo).
                            >10 hours (Escherichia coli, in vivo).


Instability index:

The instability index (II) is computed to be 22.69
This classifies the protein as stable.



Aliphatic index: 89.66

Grand average of hydropathicity (GRAVY): -0.130