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Welcome to the SIB BLAST Network Service
If results of this search are reported or published, please mention that
the computation was performed at the SIB using the BLAST network service.
The SIB BLAST network service uses a server developed at SIB and the NCBI
BLAST 2 software. It is implemented on hardware provided by HP.
In case of problems, please contact us.
NCBI BLAST program reference [PMID:9254694]:
Altschul S.F., Madden T.L., Schäffer A.A., Zhang J., Zhang Z., Miller W.,
Lipman D.J. Gapped BLAST and PSI-BLAST: a new generation of protein
database search programs. Nucleic Acids Res. 25:3389-3402(1997).
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Query length: 290 AA (of which 10% low-complexity regions filtered out)
Date run: 2003-05-01 14:59:38 UTC+0100 on sib-blast.unil.ch
Program: NCBI BLASTP 2.2.5 [Nov-16-2002]
Database: XXswissprot Viruses
8482 sequences; 3,552,911 total letters
Swiss-Prot Release 41.6 of 30-Apr-2003
List of potentially matching sequences
Alignments
| sp | P59641
R1AB_CVHSA | Replicase polyprotein 1ab (pp1ab) (ORF1AB) [Includes: Replicase
polyprotein 1a (pp1a) (ORF1A)] [Contains: Leader protein;
p65 homolog; Papain-like proteinase (EC 3.4.24.-) (NSP1);
3C-like proteinase (EC 3.4.24.-) (3CL-PRO) (NSP2); HD2
(NSP3); NSP4; NSP5; NSP6; Growth factor-like (NSP7);
RNA-directed RNA polymerase (EC 2.7.7.48) (RdRp) (NSP9);
Helicase (Hel) (NSP10); NSP11; NSP12; NSP13] [Human
coronavirus (strain SARS) (HCoV-SARS)] | 7073 AA
align |
Score = 513 bits (1320), Expect = e-146
Identities = 261/290 (90%), Positives = 261/290 (90%)
Query: 1 GKFKKIVKGTHHWMXXXXXXXXXXXVQSTQWSLFFFVYENAFLPFTLGIMAIAACAMLLV 60
GKFKKIVKGTHHWM VQSTQWSLFFFVYENAFLPFTLGIMAIAACAMLLV
Sbjct: 3547 GKFKKIVKGTHHWMLLTFLTSLLILVQSTQWSLFFFVYENAFLPFTLGIMAIAACAMLLV 3606
Query: 61 KHKHAFLCLFLLPSLATVAYFNMVYMPASWVMRIMTWLELADTSLSGYRLKDCVMYASAL 120
KHKHAFLCLFLLPSLATVAYFNMVYMPASWVMRIMTWLELADTSLSGYRLKDCVMYASAL
Sbjct: 3607 KHKHAFLCLFLLPSLATVAYFNMVYMPASWVMRIMTWLELADTSLSGYRLKDCVMYASAL 3666
Query: 121 VLLILMTARTVYDDAARRVWTLMNVITLVYKVYYGNALDQAISMWALVISVTSNYSGVVT 180
VLLILMTARTVYDDAARRVWTLMNVITLVYKVYYGNALDQAISMWALVISVTSNYSGVVT
Sbjct: 3667 VLLILMTARTVYDDAARRVWTLMNVITLVYKVYYGNALDQAISMWALVISVTSNYSGVVT 3726
Query: 181 TIMFLARAIVFVCVEYYPLLFITGNTLQCIMLVXXXXXXXXXXXXXXXXXXNRYFRLTLG 240
TIMFLARAIVFVCVEYYPLLFITGNTLQCIMLV NRYFRLTLG
Sbjct: 3727 TIMFLARAIVFVCVEYYPLLFITGNTLQCIMLVYCFLGYCCCCYFGLFCLLNRYFRLTLG 3786
Query: 241 VYDYLVSTQEFRYMNSQGLLPPKSSIDAFKLNIKLLGIGGKPCIKVATVQ 290
VYDYLVSTQEFRYMNSQGLLPPKSSIDAFKLNIKLLGIGGKPCIKVATVQ
Sbjct: 3787 VYDYLVSTQEFRYMNSQGLLPPKSSIDAFKLNIKLLGIGGKPCIKVATVQ 3836
|
|
|
| sp | P19751
RRPA_CVMJH | RNA-directed RNA polymerase (ORF1A) (EC 2.7.7.48) [Murine coronavirus
MHV (strain JHM)] | 4488 AA
align |
Score = 127 bits (320), Expect = 2e-30
Identities = 86/293 (29%), Positives = 146/293 (49%), Gaps = 10/293 (3%)
Query: 2 KFKKIVKGTHHWMXXXXXXXXXXXVQSTQWSLFFFVYENAFLPFTLGIMAIAACAMLLVK 61
K +++KGT W+ +W++F +V + L TL + AMLL+K
Sbjct: 3655 KRTRVIKGTCCWILASTFLFCSIISAFVKWTMFMYVTTH-MLGVTLCALCFVIFAMLLIK 3713
Query: 62 HKHAFLCLFLLPSLATVAYFNMVYMPASWVMRIMT--WLELADTSLSGYRLKDCVMYASA 119
HKH +L ++++P L T+ Y N + + R + WL ++ Y D V+Y
Sbjct: 3714 HKHLYLTMYIMPVLCTLFYTNYLVVGYKQSFRGLAYAWLSYFVPAVD-YTYMDEVLYGVV 3772
Query: 120 L-VLLILMTARTVYDDAARRVWTLMNVITLVYKVYYGNALDQAISMWALVISVTSNYSGV 178
L V ++ +T R++ D ++ + +++LV Y+G L++ + ++ + T +
Sbjct: 3773 LLVAMVFVTMRSINHDVFSTMFLVGRLVSLVSMWYFGANLEEEVLLFLTSLFGTYTW--- 3829
Query: 179 VTTIMFLARAIVFVCVEYYPLLFITG-NTLQCIMLVXXXXXXXXXXXXXXXXXXNRYFRL 237
TT++ LA A V +L+ T ++ ++L N FR+
Sbjct: 3830 -TTMLSLATAKVIAKWLAVNVLYFTDIPQIKLVLLSYLCIGYVCCCYWGVLSLLNSIFRM 3888
Query: 238 TLGVYDYLVSTQEFRYMNSQGLLPPKSSIDAFKLNIKLLGIGGKPCIKVATVQ 290
LGVY+Y +S QE RYMN+ GL PP++S +A LN KLLGIGG P I+V+ +Q
Sbjct: 3889 PLGVYNYKISVQELRYMNANGLRPPRNSFEALMLNFKLLGIGGVPVIEVSQIQ 3941
|
|
|
| sp | Q05002
R1AB_CVH22 | Replicase polyprotein 1ab (pp1ab) (ORF1AB) [Includes: Replicase
polyprotein 1a (pp1a) (ORF1A)] [Contains: Protein p9;
Papain-like proteinase 1 (EC 3.4.24.-); Papain-like
proteinase 2 (EC 3.4.24.-); 3C-like proteinase (EC
3.4.24.-) (3CL-PRO); RNA-directed RNA polymerase (EC
2.7.7.48) (RdRp); Helicase (Hel)] [Human coronavirus
(strain 229E) (HCoV-229E)] | 6758 AA
align |
Score = 91.7 bits (226), Expect = 1e-19
Identities = 70/248 (28%), Positives = 115/248 (46%), Gaps = 10/248 (4%)
Query: 44 PFTLGIMAIAACAMLLVKHKHAFLCLFLLPSLATVAYFNMVYMPASWVMRIMTWLELADT 103
PF + ++A++ C +VKHK FL +FLLPS+ A N + V +++ +
Sbjct: 3308 PFMILLVALSLCLTFVVKHKVLFLQVFLLPSIIVAAIQNCAW--DYHVTKVLAEKFDYNV 3365
Query: 104 SLSGYRLKDCVMYASALVLLILMTARTVYDDAARRVWTLMNVITLVYKVYYGNALDQAIS 163
S+ ++ V L + +L T R + L ++I ++Y Y +S
Sbjct: 3366 SVMQMDIQGFVNIFICLFVALLHTWRFAKERCTHWCTYLFSLIAVLYTALYSY---DYVS 3422
Query: 164 MWALVISVTSNYSGVVTTIMFLAR-AIVFVCVEYYPLLFITGNTLQCIMLVXXXXXXXXX 222
+ +++ SN + I + R + F+ VEY + ++ ++L
Sbjct: 3423 LLVMLLCAISNEWYIGAIIFRICRFGVAFLPVEYVSYF----DGVKTVLLFYMLLGFVSC 3478
Query: 223 XXXXXXXXXNRYFRLTLGVYDYLVSTQEFRYMNSQGLLPPKSSIDAFKLNIKLLGIGGKP 282
NR+ + TLGVYD+ VS EF+YM + GL P DA L+ KL+GIGG
Sbjct: 3479 MYYGLLYWINRFCKCTLGVYDFCVSPAEFKYMVANGLNAPNGPFDALFLSFKLMGIGGPR 3538
Query: 283 CIKVATVQ 290
IKV+TVQ
Sbjct: 3539 TIKVSTVQ 3546
|
|
|
| sp | P27920
VGF1_IBVB | F1 protein [F1] [Avian infectious bronchitis virus (strain Beaudette)
(IBV)] | 3951 AA
align |
Score = 54.3 bits (129), Expect = 2e-08
Identities = 26/59 (44%), Positives = 37/59 (62%)
Query: 232 NRYFRLTLGVYDYLVSTQEFRYMNSQGLLPPKSSIDAFKLNIKLLGIGGKPCIKVATVQ 290
N+ F LTLG Y++ VS ++RYM + PPK+ + F NI + GIGG + +ATVQ
Sbjct: 3321 NKVFGLTLGKYNFKVSVDQYRYMCLHKINPPKTVWEVFSTNILIQGIGGDRVLPIATVQ 3379
|
|
|
Database: XXswissprot Viruses
Posted date: Apr 29, 2003 11:07 AM
Number of letters in database: 3,552,911
Number of sequences in database: 8482
Lambda K H
0.331 0.140 0.437
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,779,133
Number of Sequences: 8482
Number of extensions: 60412
Number of successful extensions: 166
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 156
Number of HSP's gapped (non-prelim): 5
length of query: 290
length of database: 3,552,911
effective HSP length: 91
effective length of query: 199
effective length of database: 2,781,049
effective search space: 553428751
effective search space used: 553428751
T: 11
A: 40
X1: 15 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.9 bits)
S2: 55 (25.8 bits)