The Putative Secondary Structure of the putative coronavirus nsp12
 
1.氨基酸组成:(%)
%A: 5.8 %C: 1.4 %D: 6.9 %E: 6.4 %F: 5.2 %M: 1.7 %N: 4.6 %P: 3.8 %Q: 3.8 %R: 2.6
%G: 6.7 %H: 2.0 %I: 7.5 %K: 7.2 %L: 8.1 %S: 6.7 %T: 6.1 %V: 9.5 %W: 0.9 %Y: 3.2
 
2.Helix,Sheet等的预测结果:
方法:向The PredictProtein server提交多肽序列.预测实现方法参见Rost B(1996)
结果:结果包括两个部分,Fig1为图象化以后的结果图,直接的预测结果请参看这里
Fig1.Helix,Strand预测结果
 
3.Motif预测:
方法:在prosit中通过序列比对,找到蛋白中可能含有的序列的motif.
结果:下表中列出了改序列中可能含有的motif.其中,数字表示motif的第一个氨基酸在整个序列中的位置,后面的字母表示氨基酸序列.
 
N-glycosylation site
Casein kinase II phosphorylation site
Protein kinase C phosphorylation site
45 NKTT
 33 TKVD
103 TMTD
207 SQME
293 SVID		 147 SVK
171 SVK
274 TVK
287 SSK
N-myristoylation site cAMP- and cGMP-dependent protein kinase phosphorylation site
  
76 GVDIAA
140 GVLITE
146 GSVKGL
243 GQLGGL
286 GSSKCV
 109 KKPT
  
 
 
参考文献:
1.PHD: predicting one-dimensional protein structure by profile-based neural networks.
2.Prediction of protein secondary structure at better than 70% accuracy
3.Topology prediction for helical transmembrane proteins at 86% accuracy
4.The PROSITE database, its status in 1999.