Gene Expression Regulation

Targeting gene expression to specific cells or tissues is currently an intensive area of gene transfer research. One of the largest hurdles accompanying gene therapy is the inability to target genes or their expression into specific cells at sufficient quantities.

Particular gene therapy applications might require regulated gene expression system. These systems have become known as 'gene switches' and the ultimate switch for gene therapy should meet the following criteria:

Transgene activated only upon administration or removal of an exogenous compound.

The exogenous compound should be non-toxic and preferably orally administered.

The exogenous compound only activates the transgene and does not interfere with endogenous gene expression.

The following articles are sure to tell you a lot about the Expression Regulation in Gene Therapy:

Regulated gene expression system    T.Clackson

Delivering tissue regeneration  W.Mark Saltzman

Genomic integration and gene expression by a modified adenoviral vector

Changyu Zheng,  Bruce J. Baum,  Michael J. Iadarola & Brian C. O'Connell

Expression of ribozymes in gene transfer systems to modulate target RNA level
                Peter J Welch, Jack R Barber, Flossie Wong-Staal

Gene silencing as a threat to the success of gene therapy      Timothy H. Bestor