Rnai



RNAi(2004)
众所周知,RNA在生命活动中具有重要的作用，它和蛋白共同负责基因的表达和表达过程的调控。近年来研究表明,一些小的双链RNA可以高效、特异的阻断体内基因表达，促进RNA降解，可在细胞内发挥基因敲除的作用，这个过程称为RNA干预（即RNAi）。RNA干扰（RNA interference or RNAi）是一个自然存在的以双链RNA为中介降解具有相同序列RNA的机制。目前，RNAi迅速成为一个研究基因功能、发病机理、疾病防治和药物筛选的日益重要的手段。从2002年起连续被评为当今生物领域的明星分子。为此我们曾在2002、12（特别报导——70）和2002、6（特别报导——41）作了特别报导，2004年RNAi又取得了重大进展。为此CMBI集中搜集了2004年RNAi的一些重要综述和论文，供大家参考。更多文献,请用RNAi关键词通过CMBI文献查询获取。
Rnai-suppresses polyglutamine-induced neurodegeneration Rnai-quashes polyQ Rnai-RNAi Delivered across the Blood-Brain Barrier Rnai-RNA interference demonstrates a novel role for H2A.Z in chromosome segregation Rnai-Kinetic analysis of the RNAi enzyme complex Rnai-Cre-lox-regulated conditional RNA interference from transgenes Rnai-Special issue on RNA interference Rnai-Optimizing RNA interference for application in mammalian cells Rnai-Mammalian RNAi vector Rnai-inf-Interference of hepatitis A virus replication by small interfering RNAs Rnai-Independent Heterochromatin Nucleation by the Stress-Activated ATF-CREB Family Proteins Rnai-RNA interference Rnai-IMPROVING THE EFFICIENCY OF RNA INTERFERENCE IN MAMMALS Rnai-CRE recombinase-inducible RNA interference mediated by lentiviral vectors Rnai-Transposon-based RNAi delivery system for generating knockdown cell lines Rnai-Small interfering RNA production by enzymatic engineering of DNA Rnai-RNAi and Viral Gene Silencing-Use of siRNAs to prevent and treat influenza virus infection Rnai-RNAi and Viral Gene Silencing-Silencing structural and nonstructural genes in baculovirus by RNA interference Rnai-RNAi and Viral Gene Silencing-RNA silencing of rotavirus gene expression Rnai-RNAi and Viral Gene Silencing-RNA silencing in viral infections Rnai-RNAi and Viral Gene Silencing-RNA silencing-a conserved antiviral immunity of plants and animals Rnai-RNAi and Viral Gene Silencing-RNA interference, arthropod-borne viruses, and mosquitoes Rnai-RNAi and Viral Gene Silencing-RNA interference as a new biotechnological tool for the control of virus diseases in plants Rnai-RNAi and Viral Gene Silencing-RNA interference against retroviruses Rnai-RNAi and Viral Gene Silencing-Plant viral suppressors of RNA silencing Rnai-RNAi and Viral Gene Silencing-Interfering with hepatitis C virus RNA replication Rnai-RNAi and Viral Gene Silencing-Generation of an shRNAi expression library against the whole human transcripts Rnai-RNAi and Viral Gene Silencing-Derivation and function of small interfering RNAs and microRNAs Rnai-RNAi and Viral Gene Silencing-Control of nonsegmented negative-strand RNA virus replication by siRNA Rnai-RNAi and Viral Gene Silencing-by RNA Interference Rnai-RNA interference microarrays Rnai-gi-Hepatic Diseases — Hitting the Target with Inhibitory RNAs Rnai-A hypothermic-temperature-sensitive gene silencing by the mammalian RNAi Rnai-from tool to mechanism-World of small RNAs Rnai-from tool to mechanism-Tissue-specific RNA interference in post-implantation mouse embryos using directional electroporation Rnai-from tool to mechanism-RNA silencing in plants-a shortcut to functional analysis Rnai-from tool to mechanism-Managing the genome-microRNAs in Drosophila Rnai-from tool to mechanism-High-throughput RNAi in Caenorhabditis elegans Rnai-from tool to mechanism-Determinants of interferon-stimulated gene induction by RNAi vectors Rnai-from tool to mechanism- Rnai-RNA interference-mediated silencing of mutant superoxide dismutase rescues cyclosporin A-induced death Rnai-RNA interference-Human genes hit the big screen Rnai-RNA interference detected 20 years ago Rnai-Rational siRNA design for RNA interference Rnai-Knockdown by RNAi-proceed with caution Rnai-Interferon induction by siRNAs and ssRNAs synthesized by phage polymerase Rnai-A resource for large-scale RNA-interference-based screens in mammals Rnai-A large-scale RNAi screen in human cells identifies new components of the p53 pathway Rnai-The Effect of RNA Interference Down-regulation of RNA Editing 3'-Terminal Uridylyl Transferase (TUTase) 1 on Mitochondrial de Novo Protein Synthesis Rnai-Short interfering RNAs can induce unexpected and divergent changes in the levels of untargeted proteins in mammalian cells Rnai-Inducible, reversible, and stable RNA interference in mammalian cells Rnai-Enzymatic production of RNAi libraries from cDNAs Rnai-Caveolin-1 Knockdown by Small Interfering RNA Suppresses Responses to the Chemokine Monocyte Chemoattractant Protein-1 by Human Astrocytes Rnai-ns-BACE1 Suppression by RNA Interference in Primary Cortical Neurons Rnai-Transgenic RNAi Reveals Essential Function for CTCF in H19 Gene Imprinting Rnai-inf-Recognition of small interfering RNA by a viral suppressor of RNA silencing Rnai-An approach to genomewide screens of expressed small interfering RNAs in mammalian cells Rnai-The crystal structure of the Argonaute2 PAZ domain reveals an RNA binding motif in RNAi effector complexes Rnai-Unlocking the money-making potential of RNAi Rnai-Spreading silence Rnai-sex peptide of Drosophila melanogaster-Female post-mating responses analyzed by using RNA interference Rnai-RNAi reveals doublecortin is required for radial migration Rnai-RNA interference-mediated knockdown of a GATA factor reveals a link to anautogeny Rnai-MicroRNAs and small interfering RNAs can inhibit mRNA expression by similar mechanisms Rnai-Local gene knockdown in the brain using viral-mediated RNA interference Rnai-Killing the messenger-short RNAs that silence gene expression Rnai-Have our dreams been shattered Rnai-Free energy lights the path toward more effective RNAi Rnai-Exploring plant genomes by RNA-induced gene silencing Rnai-Dissecting HIV-1 through RNA interference Rnai-ca-New tools for functional mammalian cancer genetics Rnai-Use Of Rna Interference-Mediated Gene Silencing And Adenoviral Overexpression To Elucidate The Roles Of Akt-Protein Kinase-Isoforms In Insulin Actions Rnai-Small Interfering Rnas Expressed From-Pol Iii Promoter Suppress The Ews-Fli-1 Transcript In An Ewing Sarcoma Cell Line Rnai-Small Interfering Rna Inhibits Hepatitis B Virus Replication Rnai-Rnai Microarray Analysis In Cultured Mammalian Cells Rnai-Rnai As-Gene Therapy Approach Rnai-Rna Interference-On The Road To An Alternate Therapeutic Strategy Rnai-Rna Interference May Be More Potent Than Antisense Rna In Human Cancer Cell Lines Rnai-Rna Interference Is-Functional Pathway With Therapeutic Potential In Human Myeloid Leukemia Cell Lines Rnai-Rna Interference Gene Therapy-Rna Interference Gets Infectious Rnai-Rna Interference Gene Therapy-Allelic Gene Inactivation Rnai-Rna Interference For Treating Cancers Caused By Viral Infection Rnai-Modulation Of Gene Expression By Lentiviral-Mediated Delivery Of Small Interfering Rna Rnai-Maintenance Of Heterochromatin By Rna Interference Of Tandem Repeats Rnai-Inhibition Of Virus Replication By Rna Interference Rnai-High-Throughput Selection Of Effective Rnai Probes For Gene Silencing Rnai-Cytotoxic Ribonucleases And Rna Interference (Rnai) Rnai-Adenoviral Vectors Expressing Sirnas For Discovery And Validation Of Gene Function Rnai-A Story Of Mice And Men Rnai-A Micrococcal Nuclease Homologue In Rnai Effector Complexes Rnai-Analysis Reveals An Unexpected Role For Topoisomerase Ii In Chromosome Arm Congression To A Metaphase Plate Rnai-Rna Interference-Mediated Reduction In Glut1 Inhibits Serum-Induced Glucose Transport In Primary Human Skeletal Muscle Cells Rnai-Induction Of An Interferon Response By Rnai Vectors In Mammalian Cells Rnai-Hiv-Rna Interference-Potentials For The Prevention Of Hiv Infections And The Challenges Ahead Rnai-Effective Small Interfering Rnas And Phosphorothioate Antisense Dnas Have Different Preferences For Target Sites In The Luciferase Mrnas Rnai-Transgenic RNA interference in ES cell–derived embryos recapitulates a genetic null phenotype Rnai-specifics of small interfering RNA specificity Rnai-Specificity of short interfering RNA determined through gene expression signatures Rnai-RNA interference-Producing decaffeinated coffee plants Rnai-Insulin signaling through Akt-protein kinase B analyzed by small interfering RNA-mediated gene silencing Rnai-inf-Inhibition of hepatitis B virus in mice by RNA interference Rnai-Genomewide view of gene silencing by small interfering RNAs Rnai-Expression profiling reveals off-target gene regulation by RNAi Rnai-Caspase 8 small interfering RNA prevents acute liver failure in mice Rnai-The specifics of small interfering RNA specificity Rnai-The Efficacy of Small Interfering RNAs Targeted to the Type 1 Insulin-like Growth Factor Receptor (IGF1R) Is Influenced by Secondary Structure in the IGF1R Transcript Rnai-Specificity of short interfering RNA determined through gene expression signatures Rnai-Short interfering RNA-directed inhibition of hepatitis B virus replication Rnai-RNA interference-Cereal adultery Rnai-Lentivirus-delivered stable gene silencing by RNAi in primary cells Rnai-Genomewide view of gene silencing by small interfering RNAs Rnai-an ever-growing puzzle Rnai-Lentivirus-delivered stable gene silencing by RNAi in primary cells Rnai-RNA interference of influenza virus production by directly targeting mRNA for degradation and indirectly inhibiting all viral RNA transcription Rnai-RNA interference blocks gene expression and RNA synthesis from hepatitis C replicons propagated in human liver cells Rnai-Recombinant Dicer efficiently converts large dsRNAs into siRNAs suitable for gene silencing Rnai-lentivirus-based system to functionally silence genes in mammalian cells, stem cells by RNA interference Rnai-hiv-Choosing CCR5 or Rev siRNA in HIV-1 Rnai-hepatitis-siRNAs knock down hepatitis Rnai-hepatitis-RNA interference targeting Fas protects mice from fulminant hepatitis Rnai-RNA interference may be more potent than antisense RNA in human cancer cell lines Rnai-Interference of hepatitis C virus RNA replication by short interfering RNAs Rnai-A general method for gene knockdown in mice by using lentiviral vectors expressing small interfering RNA Rnai-RNA interference-traveling in the cell and gaining functions Rnai-Mammalian RNAi for the masses Rnai-Mammalian oocytes do it without RNA-dependent RNA polymerase Rnai-Systematic functional analysis of the Caenorhabditis elegans genome using RNAi Rnai-RNA interference in eukaryotic pathogens Rnai-Mammalian RNAi for the masses Rnai-Genome-wide RNAi analysis of Caenorhabditis elegans fat regulatory genes Rnai-RNA interference-traveling in the cell and gaining functions Rnai-Screening for gene function in chicken embryo using RNAi and electroporation Rnai-RNA interference machinery regulates chromosome dynamics during mitosis and meiosis Rnai-Inhibiting HIV-1 infection in human T cells by lentiviral-mediated delivery of small interfering RNA against CCR5 Rnai-Clearance of replicating hepatitis C virus replicon RNAs in cell culture by small interfering RNAs Rnai-Specific and Potent RNA Interference in Terminally Differentiated Myotubes Rnai-A systematic RNAi screen identifies a critical role for mitochondria in C. elegans longevity Rnai-RNA Interference of Signal Peptide-binding Protein SRP54 Elicits Deleterious Effects and Protein Sorting Defects in Trypanosomes Rnai-mediated silencing of Sod2 in Drosophila leads to early adult-onset mortality and elevated endogenous oxidative stress Rnai-Trypanosome Mitochondrial 3' Terminal Uridylyl Transferase (TUTase) Rnai-Tissue-specific RNA interference in postimplantation mouse embryos with endoribonuclease-prepared short interfering RNA Rnai-The dsRNA Binding Protein RDE-4 Interacts to Direct RNAi in C. elegans Rnai-Structural and functional characteristics of two sodium-coupled dicarboxylate transporters (ceNaDC1 and ceNaDC2) from Caenorhabditis elegans and their relevance to life span Rnai-Specific and potent RNA interference in terminally differentiated myotubes Rnai-Single-Stranded Antisense siRNAs Guide Target RNA Cleavage in RNAi Rnai-RNA interference of HIV replication Rnai-Regulation of Notch Signaling by O-Linked Fucose Rnai-Raptor, a Binding Partner of Target of Rapamycin (TOR), Mediates TOR Action Rnai-Inhibition of cdk2 activating phosphorylation by mevastatin Rnai-High molecular weight RNAs and small interfering RNAs induce systemic posttranscriptional gene silencing in plants Rnai-ARGONAUTE1 is required for efficient RNA interference in Drosophila embryos Rnai-A Chromosome RNAissance RNAi functions in cultured mammalian neurons Rnai-Small RNAs Correspond to Centromere Heterochromatic Repeats Rnai-Role for a bidentate ribonuclease in the initiation step of RNA interference Rnai-RNA Silencing-The Genome's Immune System Rnai-RNA Interference Rnai-Regulation of Heterochromatic Silencing and Histone H3 Lysine-9 Methylation by RNAi Rnai-Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans Rnai-Methylation of Histone H3 at Lysine 9 Targets Programmed DNA Elimination in Tetrahymena Rnai-Glimpses of a Tiny RNA World Rnai-Establishment and Maintenance of a Heterochromatin Domain, Rnai-Duplexes of 21-nucleotide RNAs mediate RNA interference in cultured mammalian cells Rnai-Dicing Up RNAs Rnai-Argonaute2, a Link Between Genetic and Biochemical Analyses of RNAi Rnai-Ancient Pathways Programmed by Small RNAs Rnai-Analysis of a piwi-Related Gene Implicates Small RNAs in Genome Rearrangement in Tetrahymena Rnai-An RNA-Guided Pathway for the Epigenome Rnai-An Extensive Class of Small RNAs in Caenorhabditis elegans Rnai-An Abundant Class of Tiny RNAs with Probable Regulatory Roles in Caenorhabditis elegans RNAi and related mechanisms and their potential use for therapy RNAi and Heterochromatin-a Hushed-Up Affair Rnai-RNA Interference Rnai-RNA Interference - A New Weapon against HIV and Beyond Rnai-Prescription RNA Rnai-Interesting Web Sites and lab Rnai-From cancer to Aids- the RNAi revolution is gathering pace Rnai-Fever Heats Up Novel Drug Category Funding Rnai-Tissue-specific RNA interference in postimplantation mouse embryos Rnai-RNA Interference Reveals a Requirement for Myocyte Enhancer Factor 2A in Activity-dependent Neuronal Survival Rnai-Gene Control by Vitamin-RNA Interactions Rnai-Retroviral delivery of small interfering RNA into primary cells Rnai-the accumulation of short interfering RNAs and micro-RNAs in tobacco Rnai-A microRNA in a Multiple-Turnover RNAi Enzyme Complex Rnai-gene-silencing in therapeutic intervention Rnai-siRNA-mediated gene silencing in vitro and in vivo Rnai-GENE SILENCING IN MAMMALS BY SMALL INTERFERING RNAS RNAi takes on DNA RNAi targeting an animal virus RNAi and Heterochromatin-a Hushed-Up Affair Rnai-Suppression of Gene Expression by RNA Interferencein Cultured Plant Cells Rnai-Single-Stranded Antisense siRNAs Guide Target RNA Cleavage in RNAi Rnai-RNA interference-listening to the sound of silence Rnai-RNA interference-antiviral defense and genetic tool Rnai-Regulation of Heterochromatic Silencing and Histone H3 Lysine-9 Methylation by RNAi Rnai-Inhibition of glioma growth by tumor-specific activation of double-stranded RNA–dependent protein kinase PKR Rnai-Efficient delivery of siRNA for inhibition of gene expression in postnatal mice Rnai-Novel Genomic cDNA Hybrids Produce Effective RNA Interference Rnai-Micro-RNAs-small is plentiful Rnai-Loss of the Putative RNA-Directed RNA Polymerase RRF-3 Makes C. elegans Hypersensitive to RNAi Rnai-Killing of leukemic cells with a BCR-ABL fusion gene by RNA interference Rnai-Inhibition of Viral Gene Expression and Replication in Mosquito Cells by dsRNA-Triggered RNA Interference Rnai-human cervical carcinoma cells treated with siRNA, a primer of RNA interference Rnai-Control of developmental timing by small temporal RNAs Rnai-Confirming Specificity of RNAi in Mammalian Cells Rnai-Combinatorial RNAi-a method for evaluating the functions of gene families Rnai-Antisense-RNA regulation and RNA interference Rnai-Targeting an Animal Virus-News from the Front Rnai-nature abhors a double-strand

Last Modified: 2004-11-28